| 1. | Es cells are the cells derived from the inner cells mass ( icm ) . they are capable of differentiating in vitro into multiple cells types under the stimulation of right signals
Es细胞来源于着床前囊胚期内细胞团( innercellmass , icm ) ，具有发育全能性。 |
| 2. | After five days of growing in culture dishes , six eggs had developed into what appeared to be blastocysts , but none clearly contained the so - called inner cell mass that yields stem cells
经过五天的培养，其中有六颗卵子发育成形似囊胚的东西，只不过没有任何一个含有可产生干细胞的所谓内细胞群。 |
| 3. | Inner cell mass ( icm ) of the blastocyst stage embryo and primordial germ cells ( pgcs ) of fetus are the mostly used materials for derivation of pluripotent stem cells
早期囊胚的内细胞团( innercellmass ， icm )和原始生殖细胞( primordialgermcells ， pgcs )是获得多能干细胞( pluripotentstemcell )的两种主要的材料来源。 |
| 4. | Purpose embryonic stem cells ( escs ) which are from the inner cell mass ( icm ) of preimplantation blastula are pluripotent . neurons are considered as terminally differentiated cells that can not divide nor proliferate after injury
目的胚胎干细胞( embryonicstemcells ， escs )来源于着床前胚胎的囊胚期内细胞团( innercellmass ， icm ) ，具有发育全能性。 |
| 5. | After 4 days of growth , the intact inner cell mass ( icm ) were separated with 0 . 05 % trypsin - edta and replated on feeder layer in dmem containing 15 % serum , 0 . 1mmol / l nonessential amino acids , 0 . 1mol / l 2 - mercaptoethanol , 2mmol / l glutamine , 100 units / ml of streptomycin and 100 units / ml of penicillin . after 4 - 6 days 5 es cell colonies were selected and expanded in which alkaline alkaline phosphatase was detected
( 2 )小鼠囊胚或内细胞的培养和es细胞的分离培养了156个不同品系的小鼠囊胚，经过3 ? 4天培养后，将增殖出来内细胞团用机械法结合胰酶- edta处理，离散后培养于mef饲养层上， 4 6天后有5例出现了es细胞样集落。 |
| 6. | We used the icr and kunming mice and got the embryos of 3 . 5dpc by means of superovulation , then cultured the embryos on the feeder layer derived from icr or kunming mice , dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time . in this period , we analized the effects of feeder layer , trypsin and embryos isolated from uteri of different varietal mice on the es cell lines
以icr小鼠和昆明小鼠为实验对象，采用超数排卵的方法获得小鼠胚胎，培养在用丝裂霉素c处理过的icr小鼠和昆明小鼠mef细胞饲养层上，选取适当时间离散增殖的icm和类es细胞集落，分析了在小鼠es细胞建系过程中，饲养层细胞、消化液及不同品系来源的胚胎的影响。 |
| 7. | Embryonic stem ( es ) cells were derived from the inner cell mass ( icm ) of preimplantation embryos , and embryonic germ ( eg ) cells from primordial germ cells ( pgc ) . both es and eg cells were cultured to determine the factors affecting on isolation , cloning and passage of pluripotent stem cells from icr mouse embryos
试验以icr小鼠胚胎内细胞团( icm )和原始生殖细胞( pgc )为材料，采用不同培养体系分离克隆胚胎多能干细胞，探讨icr品系小鼠胚胎多能干细胞分离克隆的基本方法，以及一些因素对其分离、培养、克隆、传代等效果的影响。 |
| 8. | The blastocyst was obtain from the mouse after 3 . 5 days of pregnancy , and was cultivated on the mouse embryonic flbroblast feeder layer . the blastocyst usually attach to the feeder layer after 48 hours , then the inner cell mass began to grow and form a big cell mass of embryonic stem cells . these cells can form cell clones with the conformation of embryonic stem cell
囊胚一般在48小时后贴壁并脱去透明带，由囊胚中的内细胞团增殖形成一个胚胎干细胞球，待其生长至一定程度后对其进行酶解传代，在传代后的细胞中重新生成es集落形态的细胞集落，在集落生长至一定程度后再进行传代，此后大约每5 ? 7天传代一次。 |
| 9. | The percentage of blastocysts in ml 6 medium with both taurine and edta ( 73 . 6 + 2 . 4 % ) was significantly higher than that in m16 with edta and taurine alone ( 60 + 1 . 0 % and 61 . 9 + 2 . 0 % , respectively ) . the change pattern in the cell parameters ( inner cell mass , icm ; total cell numb , tcn ) of the three groups was the same as the blastocyst formation rate
且m16 + tau + edta培养液中的囊胚总细胞数、内细胞团数、内细胞团与滋养层细胞数的比率均显著高于m16 + tau和m16 + edta培养液的( p 0 . 05 ) ；内细胞团占总细胞数的百分率也相应增加。 |
| 10. | Embryonic stem ( es ) cells are pluripotent cells derived from the inner cell mass ( icm ) of early blastrocyts , which possess the ability of unlimitted proliferation and maintain normal dipoid keptypes under differentiation - inhibited culture in vitro , they will differentiate into multiple lineage cell types or occur commentent and differentiation without differentiation - inhibited factors or hi presence of certain induced - factors .
胚胎干细胞( es细胞) ，是存在于早期胚胎内细胞团( icm )的一种多潜能细胞。在体外抑制分化培养时， es细胞可无限增殖并保持正常的二倍体核型，一旦撤除分化抑制因素，或添加适当诱导剂， es细胞可发生谱系分化或定向分化。 |
